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Tiny tweezers allow precision control of enzymes

July 3, 2013

Tweezers are a handy instrument when it comes to removing a splinter or plucking an eyebrow.

In new research, Hao Yan and his colleagues at Arizona State University’s Biodesign Institute describe a pair of tweezers shrunk down to an astonishingly tiny scale. When the jaws of these tools are in the open position, the distance between the two arms is about 16 nanometers – over 30,000 times smaller than a single grain of sand. Download Full Image

The group demonstrated that the nanotweezers, fabricated by means of the base-pairing properties of DNA, could be used to keep biological molecules spatially separated, or to bring them together as chemical reactants, depending on the open or closed state of the tweezers.

In a series of experiments, regulatory enzymes – central components in a host of living processes – are tightly controlled with the tweezers, which can switch reactions on or off depending on their open or closed condition.

“The work has important implications for regulating enzymatic function and may help usher in a new generation of nanoscale diagnostic devices as well as aid in the synthesis of valuable chemicals and smart materials,” Yan said.

Results of the new research appear in the current issue of the journal Nature Communications. Minghui Liu, a researcher in Biodesign’s Center for Single Molecule Biophysics and the Department of Chemistry and Biochemistry at ASU is the paper’s lead author. Other authors include Jinglin Fu, Yan Liu, Neal Woodbury from ASU, and Christian Hejesen and Kurt Gothelf from Aarhus University, Denmark.

Enzymes are large molecules responsible for thousands of chemical interactions essential to life. A primary role for enzymes is to accelerate or catalyze myriad chemical reactions involved in processes ranging from digestion to DNA synthesis. To do this, enzymes lower the activation energy – the minimum energy needed for chemical reactions to occur – thereby speeding up the rate of such reactions. Enzymes are critical factors for health and disease, helping cells maintain their delicate homeostasis. When mutations lead to over- or under-production in certain key enzymes, severe genetic diseases – some of them, lethal – can result.

Because of the central importance of enzymes for biological systems, researchers want to gain a better understanding of how normal enzymatic reactions occur and how they may go awry. Such knowledge may encourage the development of techniques to mimic cellular processes involved in enzyme regulation.

In the current study, the authors create a nanoscale tool designed to manipulate enzymatic reactions with fine-grained control. The group dubs their device a tweezer-actuated enzyme nanoreactor.

The clever design separates an enzyme and a cofactor essential for successful reactions on separate arms of the tweezer-like instrument. Enzyme function is inhibited when the tweezers are in their open position and the two molecules are held apart. Enzyme activation takes place when the tweezer prongs close, bringing enzyme and cofactor in contact. (The closing of the tweezers occurs when a specific DNA sequence is added, altering the thermodynamics of the system and causing a conformational change in the structure.)

The current study explores reactions in regulatory enzymes – multitasking entities that are important for modulating biochemical pathways. Regulatory enzymes, which can catalyze reactions over and over again, accomplish their feats by binding with biomolecular cofactors. (Hormone production and regulation are just one example of regulatory enzyme activity.)

In a series of experiments, the group was able to externally control the inhibition and activation of the enzyme through successive cycles. The authors stress that the nanoreactor tweezers could be used to regulate other types of enzymes and their control could be further refined by means of feedback and feed-forward loops.

Engineering nanostructures from the bottom up, using DNA as a construction material, affords researchers exacting control over the resulting geometry. Previously, Yan has created nanostructures in two- and three-dimensions, ranging from flat shapes to bowls, baskets, cages, Möbius strips and a spider-like autonomous walker.

In the tweezer design, a pair of 14 nm arms is connected at their ends by means of a 25 nucleotide single strand of DNA. This strand controls the opening and pinching of the tweezers, much the way a spring acts in a pair of gardening shears.

Two types of complementary sequence strands interact with this component, either forming a rigid DNA double helix, which supports the tweezers in their open position (set strands), or disabling the structural support and closing the tweezers (fuel strands).

Two techniques were used to measure and analyze the resulting structures with nanoscale precision: Fluorescence Resonance Energy Transfer (FRET) and Atomic Force Microscopy. Experiments demonstrated a high yield for enzyme-bound tweezers and successful switching between open and closed states was observed. The use of FRET allowed the process to be monitored in real time.

Lengthening the cofactor linker dangling from one of the tweezer’s arms enhanced successful opening and closing of the enzyme tweezers. Analysis revealed a 5-fold increase in enzymatic activity in the closed state, compared with the open state. The study also demonstrated durability in the tweezers, which were able to cycle between the open and closed positions nine times without losing structural integrity. The process was only limited by the accumulation of set strands and fuel strands.

Future work will explore similar responsive enzyme nanodevices capable of selective chemical amplification, with potentially broad impacts for medical diagnostics. Nanoreactors may also be applied as precision biocatalysts for the production of useful chemicals and smart materials.

In addition to his position in Biodesign’s Center for Single Molecule Biophysics, Hao Yan holds the Milton D. Glick Distinguished Chair in Chemistry and Biochemistry in ASU's College of LIberal Arts and Sciences.

Richard Harth

Science writer, Biodesign Institute at ASU


ASU exhibits healthier outcomes by thoughtful office design

July 8, 2013

Arizona State University’s College of Health Solutions and College of Nursing and Health Innovation are working together to create a healthier environment in support of research about health and the dangers of sedentary lifestyles in the workplace, which has been revealed by such ASU faculty members as Healthy Lifestyles director Glenn Gaesser and international obesity expert James A. Levine.

Recent studies show that office-based workers spend about 80 percent of their workday sitting, which can create health risks for office-based workers over time – including cardiovascular disease, diabetes, obesity, premature death and other risks, which exercise cannot negate. ASU employees working at their sit and stand workstations. Download Full Image

This month, team members from both colleges will be relocating to new offices at the Nursing and Health Innovation II building in Downtown Phoenix, which will inspire workplace productivity and wellness through design and collaboration. These new offices will be outfitted with height-adjustable, sit-to-stand desks and will also have three walking workstations available for use. The open layout will create a transparent and inviting environment.

“We had a few guiding principles when designing the space, which included the prevalence of natural materials and natural light, an environment conducive to interprofessionalism and collegiality, and the overall flow of the space, which is meant to be welcoming to everyone who visits,” said Melanie Burm, chief operating officer in the College of Health Solutions. “Additionally, we found that not only can these changes be a health saver, but they can actually be a cost saver too.”

The project was an opportunity for the colleges to collaborate with businesses such as Capital Projects, Smith Group, Steelcase, Target Commercial Interiors and the ASU Office of the University Architect, to develop a comprehensive solution to reduce sitting time and increase activity in the workplace. Steelcase will be evaluating workplace productivity and wellness to analyze its feasibility to implement the plan to a wider audience and impact health and productivity elsewhere.

The move also presents an opportunity for ASU students to study the impact of the new work environment on team members. Exercise and Wellness graduate student Anna Park is leading a "Sit and Stand" study, which will determine what impact – if any – standing while working and using a walking workstation has on an individual’s overall health. Research results will be ready later this year.