Of mice and men: fine-tuning salmonella-based vaccines

February 17, 2014

An innovative vaccine technology makes use of reengineered salmonella to deliver protective immunity. If such recombinant attenuated salmonella vaccines, or RASVs, can be perfected, they hold the promise of safe, low-cost, orally-administered defenses against viral, bacterial, fungal and parasitic infections.

In a new study, lead author Karen Brenneman and her colleagues at ASU's Biodesign Institute propose an improved method of screening salmonella vaccines in small animal studies and enhancing their effectiveness in humans. Salmonella Download Full Image

The new research demonstrates a system for improving the ability of salmonella vaccine strains to survive the hostile environment of the stomach, where high acid concentrations are typically lethal for invasive bacteria. The data show a tenfold improvement in salmonella survivability in a mouse model, modified to mimic stomach acid conditions in humans.

The research team – which included Crystal Willingham, Jacquelyn A. Kilbourne, Kenneth Roland and Roy Curtiss III (director of Biodesign’s Center for Infectious Diseases and Vaccinology and Professor in ASU's School of Life Sciences) – recently reported their results in the journal PLOS ONE.

“Over the years, the mouse model has taught us a lot about how Salmonella interacts with mammalian hosts.  However, it turns out that the mouse model fails to provide relevant information on how Salmonella is able to deal with the extremely low pH of the human stomach,” says Roland, corresponding author of the new study.

An RASV vaccine uses salmonella – causative agent of gastrointestinal disorders, including common food poisoning. Researchers in the Curtiss lab have been modifying salmonella by removing virulence factors that cause illness, and outfitting the bacterium with key antigens associated with the vaccine disease target.

Salmonella-based vaccines are capable of powerfully stimulating mucosal, humoral and cellular immunity against invasive pathogens, but to be effective, the vaccine cargo ships – the salmonella – must survive hostile conditions of the stomach.

While RASV results in mice have been encouraging, human trials have proven less favorable. The authors speculate that the high acid (low pH) conditions of human stomachs compared with mice may be partly responsible for the discrepancy.

Many types of salmonella exist, and the form affecting humans – Salmonella enterica serotype Typhi – is species-specific. To test RASV vaccines in vivo, prior to human clinical trials, requires the use of Salmonella Typhimurium, a closely related strain affecting mice and producing an illness similar to human typhoid.

One critical area in which mice differ from humans however, is in the acidity of their stomachs. For humans, the pH level in an empty stomach is between 1.0 and 3.0, whereas for mice, it is around 4.0.

“Finding a way to temporarily lower the stomach pH of a mouse sets the stage for further research into designing more effective oral vaccines, such as RASVs, and will provide new insights into how wild-type pathogenic bacteria survive this barrier. Our paper also demonstrates the utility of our previously described, novel acid-resistance systems to increase survival of RASVs in vivo at low pH,” Roland says.

In the new study, high acid conditions in the mouse stomach were transiently induced through the injection of histamine. The histamine mouse model was capable of distinguishing Salmonella strains showing acid sensitivity or acid resistance, previously identified under laboratory conditions. The observed results in histamine mice were shown to closely correlate with in vitro acid resistance or sensitivity.

Low gastric pH is common to many host species, including humans, and acts in part as a defense mechanism to kill the majority of ingested microbes. The microbes, however, have actively developed strategies to attempt to survive such conditions, in a kind of evolutionary arms race.

Some microbes fare better than others under low pH, however. S. Typhi – the salmonella strain used for RASVs – is normally rather intolerant of low pH conditions, compared with the S. Typhimurium strains tested in mice.

A further factor of critical importance is the difference in gastric biology between mice in humans. Prior to RASV vaccine administration, both mice and humans are usually fasted in order to empty the upper part of the gastrointestinal tract. In mice, this has the effect of raising pH from around 3.0 to 4.0. In humans however, fasting pH is lowered, falling below 2.0. As the authors note, the mouse stomach after fasting poses only a mild challenge to the RASV invasive bacteria, whereas in the human stomach, conditions are hostile enough to kill most vaccine cells, undermining effectiveness.

Bacterial cells used for RASVs are generally not cultured for acid resistance, and are therefore particularly vulnerable to high acid gastric environments, further hampering their effectiveness. One common method for getting around the vulnerability of vaccine cells is to administer them in conjunction with an antacid or other agent designed to protect from low pH. The technique improves survivability – but at a cost. Without exposure to low pH, the salmonella vaccine bacteria lose valuable positional signals that help them colonize the intestine and successfully invade host tissue.

In the current study, heightened resistance to stomach acid was conferred to RASV strains through a special system using rhamnose-regulated arginine decarboxylase. The system was shown to dramatically increase survival of acid-sensitive S. Typhi in vitro at a pH of 2.5.

The researchers next sought to examine the effectiveness of RASVs containing the new acid resistance system in vivo, under the low pH conditions induced in the histamine mouse model. Results showed a tenfold improvement in the ability of these modified strains to survive a gastric pH of 1.5 in the mouse stomach, and to successfully reach the intestinal tract. The implication is that such modified vaccine strains will likewise survive similar low pH conditions in humans, dramatically improving their effectiveness.

The study findings suggest that the histamine mouse model may be a useful tool for evaluating not only RASV candidates, but any orally administered microbial treatment (for example, pro-biotic bacteria). For microbes displaying acid sensitivity, the rhamnose-regulated arginine decarboxylase acid-resistance system can improve survivability under low pH and successful transit to the intestinal tract. Such improvement may elevate vaccine effectiveness while allowing a reduction in dose.

Richard Harth

Science writer, Biodesign Institute at ASU


Solar energy leaders come together for 4th Arizona Solar Summit

February 17, 2014

The Arizona Solar Summit brings together people and organizations to advance the solar industry on both the regional and national levels, creating a network to propel Arizona to national prominence in the industry.

The fourth annual Arizona Solar Summit, part of the 2014 Sustainability Solutions Festival, will focus on introducing innovative policies, programs and technologies that are critical to reshaping Arizona’s energy markets. Arizona Solar Summit IV Download Full Image

Participants will gain a better understanding of Arizona’s current energy market conditions, solar energy’s place in regional and national markets, and ideas for accelerating the penetration of clean technologies into this market.

What: Arizona Solar Summit IV

When: 7:30 a.m.-6 p.m., Thursday, Feb. 20

Where: ASU SkySong, 1475 N. Scottsdale Rd. RM 150, Scottsdale, 85257

Cost: $175 standard registration; $75 non-profit and community partners; $35 student registration

Registration: https://www.regonline.com/azsolarsummitIV

Media Opportunities:

Attending members of the media will have the following opportunities to engage with speakers and participants at the Arizona Solar Summit:

  • Discuss current issues at roundtable discussions
  • Capture address from keynote speaker, Bill Harris, CEO and president of Science Foundation Arizona
  • Observe multiple panel discussions with industry and thought leaders
  • Participate in industry networking sessions
  • Interact with solar energy exhibitors, including startups and research organizations
  • Interview speakers following panel discussions


Full details are available online at http://azsolarsummit.org/summit-iv-february-2014. Highlights include:

  • 7:30-9 a.m.: Roundtable discussions being led by ASU experts on a variety of renewable and solar energy topics, including policy and scientific developments at ASU, and water/energy nexus issues
  • 9:15-10:30 a.m.: Kris Mayes, director of the Utility of the Future Center, Sandra Day O’Connor College of Law professor and former chairman on the Arizona Corporation Commission moderating Panel I: Utility of the Future: How New Technology Is Bringing Change and Opportunity to Electric Companies and Their Customers. This panel features:
    • Charles Bayless, North America Energy Holdings
    • Bob Graham, Southern California Edison (retired)
    • Tim Berg, Sacramento Municipal Utility District
    • Meghan Nutting, Solar City
  • 10:45 a.m. - noon: Gary Dirks, director of the Global Institute of Sustainability at ASU and director of LightWorks moderating Panel II: De-Carbonizing the Energy System. This panel features:
    • Charles Bayless, North America Energy Holdings
    • Tim Berg, Sacramento Municipal Utility District
    • Kerry Smith, W.P. Carey School of Business, Arizona State University
    • Ellen Stechel, LightWorks, Arizona State University
  • 12:20-1 p.m.: Bill Harris, CEO and President, Science Foundation Arizona, keynote speaker
  • 1-1:15 p.m.: Sethuraman Panchanathan, senior vice president, Office of Knowledge Enterprise Development, ASU will give an address
  • 1:30-2:45 p.m.: Harvey Bryan, professor at the Design School and School of Sustainability, ASU moderating Panel III: Deep Energy Retrofit Financing. This panel features:
    • Daniel Hunter, account executive, Ameresco
    • Dimitrios Laloudakis, energy manager, City of Phoenix
    • Scott Muldavin, Rocky Mountain Institute
  • 3-4:15 p.m.: Leisa Brug, energy policy advisor to Governor Jan Brewer and director, Arizona Governor's Office of Energy Policy moderating Panel IV: National Governors Association Policy Academy Targeting Clean Energy for Economic Development and Briefing on the Arizona Master Energy Plan. This panel features:
    • Bennett Curry, Arizona Commerce Authority
    • Representative Frank Pratt, Arizona State House
    • Senator Bob Worsley, Arizona State Senate
  • 4:15-4:30 p.m.: Closing remarks by Gary Dirks and Todd Hardy, vice president of assets, ASU Foundation for a New American University and senior economic development adviser, Office of Knowledge Enterprise Development, ASU
  • 4:30-6 p.m.: Reception, Networking & Technology Showcase

Learn more at www.azsolarsummit.org.