Useful in a pinch: Nanoscale tweezers are triggered by light

New nanodevice will be useful in administering medication, treating diseases and repairing tissue

July 17, 2018

Using segments of DNA, researchers at ASU have constructed a pair of tweezers, measuring 100,000 times tinier than the width of a human hair. A brief burst of ultraviolet light causes the jaws of the tweezers to switch from their closed to open position in seconds.

The new light-activated tweezers are the latest innovation in the fast-moving world of nanotechnology. Devices of this kind, which can directly interact with and influence biological systems, are part of a new breed of nanogadgets that may ultimately be applied in drug delivery, disease treatment, tissue repair and to advance basic understanding of living systems. A three-second pulse of ultraviolet light activates tiny tweezers only a few billionths of a meter in size. The light acts to free a trigger strand from its photocage (blue dots) causing the tweezers to spring open. Above are images of the actual nanotweezers in closed and open position, made using atomic force microscopy. Image courtesy of the Stephanopoulos lab and Shireen Dooling Download Full Image

The research, which appears in the journal Angewandte Chemie International Edition, was carried out by Nick Stephanopoulos and his colleagues at the Biodesign Center for Molecular Design and Biomimetics at Arizona State University.

Stephanopoulos is a 2018 recipient of the National Science Foundation CAREER Award, the NSF’s most prestigious and competitive honor for early-career investigators.

CAREER awards support early-career faculty who have the potential to serve as academic role models in research and education and to lead advances in the mission of their department or organization. According to the NSF, “activities pursued by early-career faculty should build a firm foundation for a lifetime of leadership in integrating education and research.”

The new award will further support Stephanopoulos’ overarching research goals. The five-year, $548,000 project for the NSF (for which Stephanopoulos will act as principal investigator) is titled “Hybrid protein-DNA nanostructures and devices.”

The laser-activated tweezers described in the new study are just one example of programmable nanostructures capable of interfacing with biological systems, including living cells.

“Biological molecules such as DNA and proteins are the perfect building blocks for making nanoscale devices because they can spontaneously self-assemble into complex structures. Our job as nanotechnologists is to figure out the design rules and synthetic approaches, for making molecules that can form all sorts of interesting shapes and assemblies,” said Stephanopoulos, who is also an assistant professor in ASU's School of Molecular Scienes.

“We are like architects that select from a set of building blocks, figure out the right molecular ‘glue’ we can use to build what we want, and then analyze the properties of the things we make. Cells are full of these fascinating biomolecular nanomachines, so we are constantly ‘borrowing’ both the design principles and the molecules themselves from existing biological systems.”

Stephanopoulos works in the Biodesign Center for Molecular Design and Biomimetics directed by Hao Yan. To date, the center has successfully produced a dizzying array of two- and three-dimensional objects including sophisticated logic gatesnanorobots capable of dynamic activity and nanoarchitectures ranging from Mobius strips to silica-based structures resembling aquatic diatoms.

The new NSF project led by Stephanopoulos has three primary goals: to develop nanofibers (similar to those in tissue) composed of DNA elements linked by proteins, to design and construct three-dimensional cages mimicking viruses from both protein building blocks and DNA scaffolds, and to engineer mechanical elements like hinges or boxes with latches that are activated by proteins.

Augmenting DNA structures with protein components improves their stability and permits interactions with cells that could not be achieved with DNA alone. The proteins could also be used as actuation elements to switch the nanostructures using stimuli such as light. The resultant forms may find their way into gels to stimulate cellular repair, remotely triggered cages for releasing therapeutics to diseased cells and nanorobotic machines for a broad range of applications.

Tiniest tweezers

Scientists would like to design smart, programmable structures capable of interacting with cells and tissues. Such advances will have broad applications in disease diagnostics and treatment, engineering of new tissues and the uncovering of underlying scientific mechanisms.

Researchers coax segments of DNA to form elaborate structures at an incredibly minute scale. The technique, known as DNA origami, relies on the fact that DNA’s four nucleotides, labeled A, T, C and G, stick together in a predictable manner: A nucleotides always pair with T and C nucleotides always pair with G.

Nanoscale architects like Stephanopoulos would like to design DNA-based devices for a range of applications that can function in a stimulus-response manner. Such structures could be applied for studying receptor binding events, creating dynamic new materials such as artificial muscles or adapted as drug delivery devices.

The tweezers described in the new study are one example of a dynamic DNA nanostructure. In earlier efforts by the Yan group, segments of DNA were successfully fashioned into tweezers, which could be activated by adding a trigger strand to the aqueous environment in which the DNA nanostructure self-assembles. This trigger strand bonds to the tweezer structure, causing the jaws of the tweezers to transition from a closed to an open position, through a property known as strand-displacement. (Figure 1 shows how strand displacement can be used to add dynamic properties to DNA structures.) 

The new light-controlled tweezers, which are the first of their kind, have two key advantages. First, they open about 60 times faster than tweezers requiring an external trigger strand. Second, the new stand-alone structure could potentially be delivered into living cells and activated with light, dramatically expanding its potential applications.

Dynamic DNA nanotechnology often makes use of toehold-mediated strand displacement reactions. In this example, the red strand binds to the single-stranded toehold region on the green strand (region 1), and then in a branch migration process across region 2, the blue strand is displaced and freed from the complex. Reactions like these are used to dynamically reconfigure or assemble nucleic acid nanostructures. The design of the new tweezers takes an innovative approach, incorporating the trigger mechanism into the nanostructure itself. In this case, the trigger strand is modified with a photocage — a chemical blocking group that prevents the trigger strand from binding with its complementary sequence. The tweezers are initially held shut by a single-stranded DNA segment. Shining ultraviolet light removes the photocage and allows the trigger strand to bind its complementary sequence, which springs open the tweezers.

Richard Harth

Science writer, Biodesign Institute at ASU


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Latest graduates of Veterans Upward Bound celebrated

July 17, 2018

Annual banquet puts spotlight on veterans completing college-prep program

The latest group of veterans who've completed Arizona State University’s TRIO Veterans Upward Bound were celebrated with a banquet July 12 at the Student Pavilion on the Tempe campus.

Veterans Upward Bound is a freeThe program is supported by the U.S. Department of Education. college-preparatory program aimed at veterans in Maricopa and Pinal counties who are either low-income or potential first-generation college students. It is offered on ASU's four campuses and has individualized online courses and academic coaching, one-on-one sessions focused on skills including goal setting and financial literacy, assistance with the admissions process (whether to ASU or other colleges), and continued support throughout their academic career.

Thursday evening, the annual banquet celebrated computer literacy graduates and those eight veterans moving forward to higher education with a dinner and awards ceremony. The event included an award of recognition to ASU graduate student and academic coach Sathwika Kulamarva, a favorite of his Veterans Upward Bound students.

The evening concluded with a stole ceremony for Veterans Upward Bound and ASU alumna Vicky Yazzie, who threw up a pitchfork gesture as she walked onstage. Yazzie, who received a degree in political science, couldn't make it to her stole ceremony in May, so she chose to celebrate alongside the most recent Veterans Upward Bound alumni. 

Learn more about Veterans Upward Bound.