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ASU scientists show that a protein can be switched on and off to conduct electricity like a metal

October 27, 2017

Result, the first of its kind, could lead to a powerful new diagnostic tool in medicine

About four years ago, Arizona State University biophysicist Stuart Lindsay’s research team got a lab result that even he couldn’t quite believe. As with most scientific surprises, it goes against all conventional wisdom: the first evidence of a protein that could conduct electricity like a metal. 

It’s a result that could have important implications in medical diagnostics, but they didn’t quite accept it at first.

After years of trying to disprove the results himself and trying to account for every potential wrong avenue or detour, Lindsay and his research group have published their new findings in the advanced online edition of the Institute of Physics journal Nano Futures.

“What this paper is mainly testing out are all the alternative explanations of our data, and ruling out all of the artifacts,” Lindsay said.  

“Basically, we’ve eliminated all of those sources of 'I don’t believe this data' and we are still seeing this weird behavior of this huge protein conducting electricity. It’s still there and it’s beautiful.”

How it began

Lindsay has spent his career building new microscopes that have become the eyes of nanotechnology and next-generation, rapid and low-cost DNA and amino acid readers to make precision medicine more of a reality.

In the process, Lindsay’s research team has learned a thing or two about how single molecules behave when tethered between a pair of electrodes, which is the foundation for how his DNA readers work.

The technology, called recognition tunneling, threads single molecules down a nanopore like a thread through the eye of a needle.

As they go down the nano-rabbit hole, electrodes measure the electrical properties of these single DNA or amino acid molecules to determine their sequence identity.

Having spent a considerable amount of time building DNA and amino readers, they decided to give whole proteins a try.

“The thought was, that if you can specifically trap a whole protein between a pair of electrodes, you would have a label-free electronic reader,” said Lindsay, who serves as director of the Biodesign Institute’s Center for Single Molecule Biophysics and as Regents’ Professor with ASU’s Department of Physics and the School of Molecular Sciences.  

The potential to have a nanotechnology device sensitive enough to identify a single protein molecule could become a powerful new diagnostic tool in medicine.

But the building blocks in every cell, proteins, were thought to behave electrically as inert organic blobs. Electronically, they were assumed to act as insulators, just like putting a piece of plastic over a metal wire.

“There is just a large amount of swept-under-the-rug data on the electrical properties of proteins,” Lindsay said. “There is one camp who dismiss these claims. There is another camp that says proteins are incredible electrical conductors. And never the twain shall meet, just like American politics.”

So four years ago, one of his graduate students at the time, Yanan Zhao, tethered a protein between two electrodes, turned up the voltage, and voila! The protein started performing like a metal, with a wild and “remarkably high electronic conductance.”

“If it’s true, it’s amazing,” Lindsay said. 

Where the weirdness begins

The first remarkable results were performed with a technology Lindsay helped spearhead, called Scanning Tunnel Microscopy, or STM. A glue-like protein, called an integrin, that helps cells stick together and assemble into tissue and organs, was used in the experiment.

Extending from the tip of the STM was another electrode attached to a small molecule, called a ligand, which specifically binds to the integrin protein. Once held in place, the STM has a lever arm and probe much like a stylus and needle on a turntable to bring the ligand in contact with its integrin target.

This is where the weirdness began.

“I just didn’t believe it, because what he saw were giant pulses of current when the probe was known to be a great distance from the surface,” Lindsay said.

That gap would have been too great for the electricity to flow through by electron hopping, or tunneling, as what occurs with Lindsay’s recognition tunneling sequencing technology.

Lindsay scratched his head in vain trying to match a theory to explain the phenomena.

“That data simply cannot be explained by electron tunneling,” he said.

Kick-starting a protein

A key turning point was Lindsay uncovering the work of theoretical biophysicist Gabor Vattay at Eötvös Loránd University in Budapest, Hungary. Lindsay read a paper of his that basically suggested that an electrical fluctuation can kick-start a protein into being a great conductor or a great insulator.

“It’s just poised to do this fluctuating thing,” Lindsay said. “In our experiments, we were seeing this weird behavior in this huge protein conducting electricity, but it is not static. It’s a dynamic thing.”

The electronic spikes occurred with increasing frequency as they upped the voltage across the protein. And there is a threshold to cross.

“Below a certain bias, it’s just an insulator, but when the fluctuations start kicking in, they are huge,” Lindsay said.

“Because of this, I contacted Gabor, and he had to use some of the best supercomputers in Europe to analyze our large protein. Basically, there are three curves for the distribution of energy level spacings, one corresponding to a metallic state, another to an insulator state, and middle third, corresponding to the quantum critical state.

“Low and behold, our protein is in the quantum critical state, if you believe the theory.”

Next, Lindsay’s team was able to manufacture a nanodevice to more finely control another series of experiments, with a carefully sized gap to control the protein and the amount of voltage that can be applied to it.  

“In the device, you get this beautiful switching on and off of the electrical conductance of the protein,” Lindsay said. 

What’s next

The results have demonstrated that fundamental quantum forces are work to explain the way the integrin protein was behaving in the experiments.

It’s also upending the way scientists are viewing the electrical properties of proteins.

“There are people who are beginning to think of proteins as quantum mechanical objects,” Lindsay said.

Next, Lindsay wants to explore other medically important proteins and measure their behavior using the solid-state nanodevices.

Could proteins vital to health and disease turn out to behave like metals? Or insulators?

One thing is certain: An entirely new way of examining protein behavior has opened up new scientific vistas that previously Lindsay and many others didn’t think was possible.  

“I believe the data now, but it’s only one protein so far,” Lindsay cautioned.

And for Lindsay, a serial entrepreneur with successful ASU spin-out companies, he may have one more trick up his sleeve to translate a basic discovery into the marketplace.

In addition to Lindsay, Zhang and Vattay, the research team included Bintian Zhang, Weisi Song, Pei Pang and Peiming Zhang from ASU’s Biodesign Institute and School of Molecular Sciences, and Department of Physics and Istvan Csbai from the Department of Physics of Complex Systems, Eötvös Loránd University, Budapest, Hungary.

The work was supported by the National Human Genome Research Institute, Recognition AnalytiX and the Edward and Nadine Carson Presidential Chair fund. 

Joe Caspermeyer

Managing editor , Biodesign Institute

480-258-8972

Futurists, visionaries, creative thinkers to look into Arizona's future at Nov. 7. conference


October 27, 2017

Disruptors and innovations in the immediate future, and their impact on Arizona transportation and the workforce will be the focus of this year’s State of Our State Conference, the annual signature event of ASU Morrison Institute for Public Policy.

The Nov. 7 event will be held 8 a.m. to 1:30 p.m. at the Phoenix Airport Marriott, 1101 N. 44th Street, in Phoenix. Conference details and registration are at MorrisonInstitute.asu.edu. Futurist Brian David Johnson will be keynote speaker at Nov. 7 conference Futurist Brian David Johnson will be keynote speaker at the State of Our State Conference on Nov. 7 in Phoenix. The conference is the annual signature event of ASU Morrison Institute for Public Policy. Download Full Image

“The future is something that’s on everyone’s mind but often difficult to visualize and even more difficult to plan for. This conference and its impressive line-up of speakers and experts will offer a unique insight into tomorrow and how Arizona can better prepare for changes to our workforce, revenue base, urban form, governance structure and more,” said Andrea Whitsett, interim director at Morrison Institute. “This is a must-attend event for creative thinkers in business, government, education, nonprofits and community leadership.”

The keynote speaker will be Brian David Johnson, Arizona State University’s futurist-in-residence. Using ethno-graphic field studies, technology research, cultural history, trend data global interviews and even science fiction, Johnson and the ASU Center for Science and the Imagination work with organizations to develop a pragmatic road map to the future and an actionable 10- to 15-year vision.

“If you look at the work that I do, one of the main themes has always been the intersection between imagination, science and engineering. Imagination is the No. 1 underutilized tool in industry,” said Johnson, who is also a professor of practice in the School for the Future of Innovation in Society and was a futurist at Intel Corp. before joining ASU.

Johnson remains a futurist and fellow at Frost and Sullivan, a visionary innovation company that’s focused on growth. He has more than 30 patents and is author of a number of books of fiction and nonfiction, including "Science Fiction Prototyping"; "Screen Future: The Future of Entertainment, Computing and the Devices We Love"; "Humanity and the Machine: What Comes After Greed?"; and "Vintage Tomorrows: A Historian and a Futurist Journey Through Steampunk into the Future of Technology."

The conference is intended to be imaginative yet pragmatic, focusing on the near future in real time — not 100 years from now. “If you look too far out it gets fuzzy,” Johnson said.

Guest speaker will be award-winning Orly Lobel, the Don Weckstein Professor of Labor and Employment Law at the University of San Diego, where she teachers and writes in the areas of employment law, intellectual property law, regulatory and administrative law, torts, behavioral economics, health policy, consumer law and trade secrets.

Lobel’s books include “Talents Wants to Be Free: Why We Should Learn to Love Leaks, Raids and Free Riding.” She currently is writing a new book, “You Don’t Own Me,” about innovation battles and how policy has shaped the dynamics at play in the toy industry.

Morrison Institute, which is commemorating its 35th anniversary, also will honor two new Distinguished Fellows: Denise D. Resnik, a leader in autism research and community awareness, and Gary L. Trujillo, an entrepreneur who has expanded leadership and business markets for the Latino community.

 Other conference speakers and panelists include:

• Kirk Adams, chief of staff, Office of the Governor
• Eric Anderson, transportation director, Maricopa Association of Governments
• Diana Bowman, associate professor, ASU Sandra Day O'Connor College of Law and the School for the Future of Innovation in Society
• Jaime Casap, education evangelist, Google
• Carlos Contreras, regional public affairs director, Intel Corp.
• Rena Davis, public policy manager, Lyft
• Jonathan Koppell, dean, ASU College of Public Service and Community Solutions
• Tekedra Mawakana, global head of policy and government affairs, Waymo
• Duke Reiter, senior advisor to ASU President Michael Crow
• Luke Tate, assistant vice president and executive director of Opportunity Initiatives and professor of practice in the School for the Future of Innovation in Society, ASU

For more information and complete list of speakers, go to MorrisonInstitute.asu.edu